KMID : 0895820120220020047
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Journal of Oriental Rehabilitation Medicine 2012 Volume.22 No. 2 p.47 ~ p.66
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Anti-inflammation and Anti-obesity Effects of Euiiin-tang Granules on High Fat Diet-Induced Obese C57BL/6J Mice
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Park Kyung-Moo
Lim Hyung-Ho Song Yun-Kyung
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Abstract
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Objectives :The study was designed to experiment anti-inflammation and anti-obesity effect of Euiiin-tang(yiyiren-tang) granules to the C57 BL/6J mice which was induced obesity by high-fat diet.
Methods :We performed a cytotoxicity analysis of Euiiin-tang by MTT assay principles. We investigated the effects of Euiiin-tang on restraining enzyme revelation of inflammation acceleration and hindering inflammation by measuring iNOS, COX-2 and NF-¥êB revelation. We treated Euiiin-tang to 3T3-L1 in Oil Red O experiment and measured a reduction effect of accumulation of fat. In order to investigate the obese inhibitory effects of Euiiin-tang, C57BL/6 mice were induced by high fat diet. C57BL/6 mice were divided into three group(normal, high fat diet, high fat diet with Euiiin-tang granules) and fed for 6weeks. The measurements have been performed on ; the body weight ; the weight of mice¡¯ liver, kidney and epididymal AT ; the quantity of AST, ALT, ¥ã-GTP, BUN, total cholesterol, HDL, LDL, plasma insulin, blood leptin.
Results :After an experiment of measuring iNOS and COX-2 revelation, Euiiin-tang showed efficacy of restraining enzyme revelation of inflammation acceleration. Treating Euiiin-tang hindered vitality of NF-¥êB, so we could identify effects of hinderance of inflammation. It was shown that accumulation of fat was significantly reduced after treating Euiiin-tang to 3T3-L1 in Oil Red O experiment. After treating Euiiin-tang in oral for 6 weeks¡¯ experiment, it was identified that it had an efficacy of restraining body weight gain. As a result of measuring the weight of mice¡¯ liver, kidney and epididymis, the weights were reduced comparing with high fat diet group, so it came out that it has prevention effect in accumulation of fats. The AST, ALT, ¥ã-GTP, BUN levels were lower in group of Euiiin-tang than that of high fat diet group. In terms of blood lipids, it was verified that total cholesterol level was in normal range with group of Euiiin-tang and HDL cholesterol level was higher than that of the high fat diet group. While concentration of plasma insulin was lower in group of Euiiin-tang than that of high fat diet group. Also the blood leptin concentration was lower in group of Euiiin-tang than that of high fat diet group.
Conclusions :From all these results, it can be concluded that the Euiiin-tang showed a significant efficacy as obesity treatment and it is thought to be a safe treatment that we could expect even the efficacy of anti-inflammatory.
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KEYWORD
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Obesity, Euiiin-tang, Anti-inflammation, Anti-obesity
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